asper venom, even when administered prior to envenomation.
On the other hand, polyvalent antivenom did not interfere with hyperalgesia or edema induced by B. jararaca venom was neutralized only when bothropic antivenom was administered iv 15 min before venom injection, whereas edema was neutralized when antivenom was injected 15 min or immediately before venom injection. When neutralization assays involving the independent injection of venom and antivenom were performed, the hyperalgesia induced by B. Both venoms also induced edema with a similar time course. asper venoms caused hyperalgesia, which peaked 1 and 2 h after injection, respectively. jararaca venom and polyvalent antivenom produced at Instituto Clodomiro Picado (AVCP, 1 ml neutralizes 2.5 mg B. asper venoms was studied in rats using bothropic antivenom produced at Instituto Butantan (AVIB, 1 ml neutralizes 5 mg B. Considering the reduced number of wild-caught snakes donated to Butantan Institute in the last decades, and the increased life expectancy of the snakes raised in captivity in the Laboratory of Herpetology, this work focused on the comparativeĮvaluation of antivenoms in the neutralization of hyperalgesia and edema induced by Bothrops jararaca and Bothrops asper snake venomsĭirectory of Open Access Journals (Sweden)įull Text Available Neutralization of hyperalgesia induced by Bothrops jararaca and B. Its venom shows individual, sexual, and ontogenetic variability, however, the effect of animal captivity upon venom composition is unknown. Bothrops jararaca is responsible for the majority of snakebites in South and Southeastern Brazil. Snakebite envenomation is a neglected tropical pathology whose treatment is based on the use of specific antivenoms. jararaca venom from captive specimens in the composition of BBRV would not interfere with the quality of this reference venom. Considering these results we suggest that the inclusion of B. jararaca venom from captive specimens and BBRV showed similar values.
Lethality and neutralization of lethality for B. Similar cross-reactivity recognition pattern of both venoms by the antibothropic antivenom produced by Butantan Institute was observed. Comparison of enzymatic activities showed minor differences between the two venoms. Electrophoretic analysis and proteomic profiling revealed few differences in venom protein bands and some differentially abundant toxins. jararaca venom from captive specimens and BBRV in order to understand the potential effects of snake captivity upon the venom composition. Considering these facts, we compared the biochemical and biological profiles of B. Nevertheless, in the last decades a high number of Bothrops jararaca specimens have been raised in captivity in the Laboratory of Herpetology of Butantan Institute. A significant reduction of the number of snake donations to Butantan Institute (São Paulo, Brazil) occurred in recent years, and this fact may impair the production of the Brazilian Bothropic Reference Venom (BBRV). Snake venom is a variable phenotypic trait, whose plasticity and evolution are critical for effective antivenom production. Functional and proteomic comparison of Bothrops jararaca venom from captive specimens and the Brazilian Bothropic Reference Venom.įarias, Iasmim Baptista de Morais-Zani, Karen de Serino-Silva, Caroline Sant'Anna, Sávio S Rocha, Marisa M T da Grego, Kathleen F Andrade-Silva, Débora Serrano, Solange M T Tanaka-Azevedo, Anita M
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